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KMID : 0613820130230121420
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Journal of Life Science
2013 Volume.23 No. 12 p.1420 ~ p.1427
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Synergistic Effect of Acetylalginate Esterase and Alginate Lyase on the Degradation of Acetylalginate from Pseudomonas aeruginosa ATCC 39324
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Kim Hee-Sook
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Abstract
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A novel acetylalginate esterase (AcAlgE) gene was previously cloned and characterized from Sphingomonas sp. MJ-3. In this study, the synergistic effects of MJ-3 AcAlgE, and KS-408 alginate lyase on the degradation of acetylalginate from Pseudomonas aeruginosa were investigated by using high-field 1H-NMR and an FPLC-equipped peptide column. The alginate lyase coupled assay of AcAlgE showed that degradation of high molecular weight acetylalginate was more difficult than degradation of acid hydrolyzed acetylalginate. The degradation of acetylalginate by alginate lyase was easier after AcAlgE was used to remove the acetyl group from acetylalginate. This result showed that the recombinant AcAlgE enhanced the degradation of acetylalginate by alginate lyase.
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KEYWORD
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Acetylalginate esterase (AcAlgE), alginate lyase, acetylalginate, alginate lyase coupled assay
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